![]() ![]() In the environment, Cu is widely used as a pesticide against fungal and bacterial diseases in crops or as a contaminant in organic amendments such as pig manure or sewage sludge ( Tabatabai, 1977 Christie & Beattie, 1989). Microcosm experiments detected the microbial community modifications with greater precision in the short-term, while field experiments showed that the biological effects of Cu contamination may be overcome or hidden by pedo-climatic variations.įield experiment, microcosm experiment, microbial community, Cu impact Introduction ![]() These modifications were transient in all cases, suggesting a short-term effect of Cu stress. ARISA fingerprinting showed slight but significant modifications of bacterial and fungal communities in field and microcosm incubation. In the field study, no significant modifications were observed in C-biomass whereas microcosm incubation showed a decrease in B3 contamination only. Quantitative modifications of the microflora were assessed by C-biomass measurements and qualitative modifications were assessed by the characterization of the genetic structure of bacterial and fungal communities from DNA directly extracted from the soil, using B- and F-ARISA (bacterial and fungal automated ribosomal intergenic spacer analysis). Furthermore, total Cu and Cu-EDTA declined with time in Au soil, whereas they remained stable in Ep soil. More extractable Cu was observed in sandy soil (Au) than in silty soil (Ep). Cu was added as ‘Bordeaux mixture’ at the standard rate used in viticulture (B1 = 16 kg Cu kg −1 soil) and at a higher level of contamination (B3=48 kg Cu ha −1 soil). The effects of Cu amendment on indigenous soil microorganisms were investigated in two soils, a calcareous silty clay (Ep) and a sandy soil (Au), by means of a 1-year field experiment and a two-month microcosm incubation. ![]()
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